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. 2011 Jun 17;6(6):e21213. doi: 10.1371/journal.pone.0021213

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Demilly et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Stage 15 embryos stained with Col and different motor neuron markers. (A–B″) No overlap between Col and pMad is found in any of the different regions of the VNC examined. (C–C″) Col and Zfh1 expression only overlaps in the dorsal apterous neuron (dAp). (D–D″) Col is only found expressed in one Islet-tau-mycEGFP+ interneuron (see also Figure 3) that we have named dCID (dorsal Col, Islet, Dachshund) neuron and excluded from the identifiable Islet+ motor neurons RP1–5. (E–E″) Expression of Col and HB9^Gal4 is mutually exclusive. (F–G″) Col is not expressed by the Eve+ aCC, RP2 and U1–5 motor neurons neither by the pCC interneuron. Arrowheads: RP2 motor neuron; arrows: aCC motor neuron; arrows in (C–D″) indicate dCID interneuron; asterisks: U/CQ motor neurons (U1–5); brackets: RP1, 3, 4, 5 motor neurons; double arrowheads: pCC interneuron.