Figure 5.

Growth characteristics of transfected cells. Vector- and HOXB7-transfected IOSE-29 cells were seeded at 2,000 cells per well in uncoated 96-well plates (A and B) and wells coated with poly(HEMA) (C). Total numbers of cells in each uncoated well were counted daily (A). After 1, 2, 3, and 4 days in culture, thymidine incorporation was measured in cells pulsed with [³H]methylthymidine for 3 h in uncoated wells (B), and for 18 h in poly(HEMA)-coated wells (C). Shown are the mean values of three to four independent experiments. Differences in cell numbers and thymidine incorporation levels of HOXB7-transfected cells, as compared with corresponding vector-transfected cells, at each time point were found to be statistically significant (P < 0.001).