Figure 3. Foxp3-expressing iTregs have limited in vivo persistence in GVHD recipients.

(A-C) Lethally irradiated Balb/c mice were transplanted with TCD B6 BM, B6 spleen cells (adjusted to yield 0.6 × 10⁶ αβ T cells), and 0.6 × 10⁶ iTregs. Mice (n=8-12/group) were sacrificed at 7, 10, or 14 days post transplantation. (A). Representative dot plots gated on CD4⁺ cells depicting EGFP⁺ iTregs in the specified tissue sites. (B,C). Relative and absolute number of iTregs in the spleen, liver, lung and colon at the defined time points post transplantation. Data are presented as the mean ± SEM and are the cumulative results from three independent experiments. (D,E). Lethally irradiated Balb/c mice were transplanted with TCD B6 BM, B6 spleen cells (adjusted to yield 0.6 × 10⁶ αβ T cells), and 0.6 × 10⁶ nTregs. Mice (n = 4-8/group) were sacrificed at 24 or 60 days post transplantation. (D). Representative dot plots gated on CD4⁺ cells depicting EGFP⁺ nTregs in the specified tissue sites. (E). The relative percentage of nTregs in the spleen, liver, lung and pooled colon samples is shown. Data are presented as the mean ± SEM and are the cumulative results from two experiments.