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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Neurogastroenterol Motil. 2011 May 13;23(7):e294–e308. doi: 10.1111/j.1365-2982.2011.01721.x

Figure 3.

Figure 3

ODT8-SST injected intracisternally prevents the surgery-induced decrease of acyl ghrelin levels. Overnight fasted rats were injected i.c. under short isoflurane anesthesia with ODT8-SST (1 μg/rat in 10 μl ddH2O) or vehicle (10 μl ddH2O) and afterwards underwent abdominal surgery or sham procedure. Animals were placed back in their home cages without access to food and water. Blood was obtained by cardiac puncture 50 min later, processed using the RAPID method and circulating acyl (A) and total ghrelin levels assessed by radioimmunoassay. Desacyl ghrelin (B) was calculated as the difference of total minus acyl ghrelin for each individual sample. Each bar represents the mean ± sem of number of rats indicated at the bottom of the columns. * P < 0.05 vs. vehicle/sham; # P < 0.05 vs. ODT8-SST/sham; ++ P < 0.01 vs. ODT8-SST/surgery.