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. Author manuscript; available in PMC: 2011 Jun 18.
Published in final edited form as: ACS Chem Biol. 2011 Mar 23;6(6):628–635. doi: 10.1021/cb100428c

Figure 4. The IC50 of RAD51 inhibition by four selected compounds determined in the D-loop assay.

Figure 4

RAD51 (1 μM) was incubated with a 90-mer ssDNA (3 μM) (Oligo 90) to form the filament followed by addition of A03, A04, A10, and B02 inhibitors in indicated concentrations. After a 30-min incubation, D-loop formation was initiated by addition of pUC19 supercoiled dsDNA (50 μM). The DNA products were analyzed by electrophoresis in a 1% agarose gel. Experiments were repeated at least three times; error bars represent S.D.