Table 2.

Antinociceptive Effect of Prolonged Treatment with V. ashei Extract on Heat-Induced Pain in Mice (Hot Plate Test)

	Latency (minutes)
Treatment (mg/kg)	0	30	60	90	120	150
Control	7.4 ± 1.8	9.0 ± 2.5	7.9 ± 1.8	10.6 ± 2.6	9.4 ± 2.7	8.7 ± 2.2
Extract (3.2)	13.0 ± 3.0	26.2 ± 2.5*	26.4 ± 2.1*	26.5 ± 2.3*	30.0 ± 0.0*	27.67 ± 1.8*
Extract (6.4)	7.1 ± 1.4	19.0 ± 3.3*	23.7 ± 2.4*	24.0 ± 2.4*	26.6 ± 1.86*	26.9 ± 1.6*

Data are mean ± SEM for 10 animals per group. Student-Newman-Keuls analysis shows that all groups treated with both concentrations of extract (3.2 mg/kg and 6.4 mg/kg) at 30, 60, 90, 120, and 150 minutes after administration are significantly different from the control group (*P < .05).