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. Author manuscript; available in PMC: 2011 Jun 18.
Published in final edited form as: Biochem J. 2010 Dec 15;433(1):31–42. doi: 10.1042/BJ20100985

Figure 6.

Figure 6

Structure of an E3/E2~Ub complex: HECT-E3, Nedd4L (blue), in complex with UbcH5b~Ub (green/red). Left, HECT N-lobe contacts E1/E3 surface on UbcH5, as observed in previous structures (see Figure 3) and HECT C-lobe makes contacts with the ~Ub moiety. The NEDD4L active site is shown in yellow (PDB code 3JVZ). Right, view rotated by 180 degrees to show NEDD4L residues analogous to those observed in Rsp5 and SMURF2 that are important for non-covalent Ub binding in the N-lobe, shown as orange spheres.