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Western blot analysis for P-selectin in whole lung homogenates. Wild-type (vWf +/+) and vWf-deficient (vWf −/−) mice were injected intraperitoneally with LPS, and 4 h later lungs were collected and homogenized. One hundred micrograms of protein was loaded onto each lane of a 5% polyacrylamide gel. The blot was probed with a polyclonal antibody against P-selectin. Numbers indicate the migration of molecular mass markers (in kDa).
