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Vacuoles induced by MIPP acquire characteristics of late endosomes, but remain distinct from autophagosomes. U251 cells expressing GFP-Rab7 were treated with 10 μM MIPP and then processed for immunofluorescence microscopy. Confocal imaging was used to localize endogenous LAMP1, EEA1, LC3II and Rab11 (red) relative to the GFP-Rab7 (green). The scale bars are 10 microns.
