MED29 expression inhibits tumor growth in vivo. A total of 2.5 × 106 cells (MIAPaCa2/MED29, MIAPaCa2/mock, Hs700T/MED29, and Hs700T/mock) were s.c. inoculated into the flanks of nude mice (n=40) and allowed to grow for up to seven (Hs 700T) or eight (MIA PaCa-2) weeks. (a) Photographs of MIAPaCa2/MED29, Hs700T/MED29, and the corresponding mock tumors taken after the 7–8 weeks of growth in nude mice. The black scale bar corresponds to 1 cm. (b) Tumor diameters were measured twice a week and the growth curves of the Hs 700T and MIA PaCa-2 tumors were generated according to the formula V=(π/6)(d1×d2)3/2, where d1 and d2 are perpendicular tumor diameters. Average tumor volumes +/− s.e.m. are shown.