Table 2.
Pst DC3000 resistant lines with one cDNA insert
| Sequence in comparison with genomic DNA§ | Response to | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Line No | Original line | Independently transformed line(s)* | Accession no.† | RAP ID‡ | RAP description‡ | cDNA | Protein | C. higginsianum¶ | Xoo** | Pst counts†† |
| Resistance confirmed in multiple independent line(s) (19 lines) | ||||||||||
| 1 | K00714 | RT | AK068846 | Os01t0127300-01 | SufBD family protein | Ok | Ok | S | S | |
| 2 | K21617 | RT | AK103699 | Os10t0530900-01 | Similar to Glutathione S-transferase GST 30 | 1 ins | Ok | R | S | |
| 3 | K00841 | RT | AK072201 | Os01t0503400-04 | Similar to metal transporter Nramp6 | 1 del | 62% shorter | R | S | Yes |
| 4 | K15424 | R06015, RT | AK070024 | Os09t0533600-01 | Similar to Avr9/Cf-9 induced kinase 1 | Ok | Ok | R | R | Yes |
| 5 | K04135 | K20450, RT | AK100547 | Os02t0145600-01 | Conserved hypothetical protein | Ok | Ok | S | S | |
| 6 | K25904 | K18218(2inserts) | AK072899 | Os09t0363900-01 | Similar to HOTHEAD protein precursor | Ok | Ok | R | S | |
| 7 | K02342 | K23019 | AK102525 | Os12t0619000-01 | IQ calmodulin-binding region domain containing protein | Ok | Ok | R | S | Yes |
| 8 | K21204 | K17730 | AK102125 | Os08t0250700-01 | Thioredoxin domain 2 containing protein | Ok | Ok | S | Yes | |
| 9 | K29409 | RT | AK099032 | Os03t0240500-01 | Similar to Toc34-2 protein | Ok | Ok | S | ||
| 10 | R04214 | RT | AK069592 | Os01t0232100-01 | Similar to Tonoplast membrane integral protein ZmTIP4-3 | 1 ins | Ok | S | ||
| 11 | K03301 | RT | AK070417 | Os03t0197100-01 | Similar to Sugar transporter protein | Ok | Ok | S | Yes | |
| 12 | K03216 | K18912 | AK101795 | Os04t0382300-01 | Similar to SNF1-related protein kinase regulatory gamma subunit 1 | Ok | Ok | R | ||
| 13 | K37838 | K25231 | AK070720 | Os03t0563300-03 | Similar to Mg-chelatase subunit (Fragment) | 1 bs | Ok | S | ||
| 14 | R05946 | K02535(2inserts) | AK099542 | Os06t0639550-01 | Non-protein coding transcript | Ok | no protein | S | ||
| 15 | R06202 | K04148 | AK071866 | Os03t0738300-01 | Hypothetical protein | 1 bs | Ok | S | ||
| 16 | R06736 | K13623 | AK101242 | Os04t0346800-02 | EAR repeat containing protein | 1 ins | 2× longer | S | ||
| 17 | R06848 | K13001 | AK073641 | Os06t0623600-01 | Similar to Cinnamoyl-CoA reductase | Ok | Ok | S | ||
| 18 | R03132 | K19414, K30128 | AK069465 | Os07t0622100-01 | Similar to Ribosomal protein s6 RPS6-2 | 1 bs | Ok | S | ||
| 19 | K09018 | RT | AK071510 | Os06t0556200-01 | Similar to Amino acid permease I | Ok | Ok | S | ||
| No independent lines available for confirmatory screening (16 lines) | ||||||||||
| 20 | K17110 | AK101316 | Os07t0435100-01 | Similar to 26S proteasome subunit RPN12 | 1 del 2 bs | Ok | R | |||
| 21 | K19720 | AK072674 | Os03t0333300-02 | Similar to eukaryotic translation initiation factor 2 beta subunit | 2 ins | 40% longer | R | S | ||
| 22 | K08435 | AK068205 | Os06t0661600-01 | Zinc finger, DPH-type domain containing protein. | 1 bs | Ok | S | |||
| 23 | K17109 | AK111889 | Os10t0160000-01 | Similar to Ubiquitin carboxyl-terminal hydrolase 12 | Ok | Ok | S | |||
| 24 | K04020 | AK066139 | Os09t0461700-01 | Alpha/beta hydrolase fold-3 domain containing protein | 1 bs | 1 ac | R | |||
| 25 | K37931 | AK071286 | Os01t0803300-01 | Protein of unknown function DUF6 | 1 bs | 1 ac | S | |||
| 26 | K39531 | AK099196 | Os02t0590400-02 | Lecithin:cholesterol acyltransferase family protein. | Ok | Ok | R | |||
| 27 | K40223 | AK065007 | Os01t0978100-01 | Similar to Cysteine synthase, mitochondrial precursor | Ok | Ok | S | |||
| 28 | K40946 | AK103235 | Os02t0829100-01 | Replication protein A 30 kDa | Ok | Ok | S | |||
| 29 | R04016 | AK102402 | Os02t0489400-01 | Similar to 40S ribosomal protein S8 | 2 bs | 1 ac | S | |||
| 30 | R06007 | AK103707 | Os01t0160800-01 | Similar to Protein synthesis inhibitor II (Ribosome-inactivating protein II) | 1 bs | Ok | S | |||
| 31 | R05018 | AK111775 | Os01t0313300-01 | Similar to EREBP-3 protein (Fragment) | Ok | Ok | R | |||
| 32 | K03221 | AK070873 | Os04t0103100-01 | Glycosyl transferase, family 43 protein | 2 ins 1 del 2 bs | 8.4% shorter | S | |||
| 33 | K17538 | AK070457 | Os10t0190900-01 | Multi antimicrobial extrusion protein MatE family protein. | 1 del 1 bs | 1 ac | S | |||
| 34 | K31418 | AK101216 | Os10t0573900-01 | NMD3 family protein. | 1 del 2 bs | 37% shorter | S | |||
| 35 | K30521 | AK073206 | Os10t0573900-03 | Similar to Nonsense mediated mRNA decay protein 3. | 2 del 1 bs | Ok | S | |||
*
RT represents retransformed.
†
Accession No. provided by KOME (http://cdna01.dna.affrc.go.jp/cDNA/).
‡
ID and predicted protein annotation provided by RAP-DB (http://rapdb.dna.affrc.go.jp/).
§
KOME cDNA sequences compared to genomic DNA sequences in RAP-DB. Ok, sequence identical to either genomic DNA sequence or the predicted protein sequence based on the genomic DNA data; bs, base substitution; ins, insertion; del, deletion; ac, amino acid change.
¶
Colletotrichum higginsianum on the FOX hunting lines; R, resistant; S, susceptible.
**
Xanthomonas oryzae pv oryzae on transgenic Nipponbare overexpressing the pertinent full-length rice cDNA; R, resistant, S, susceptible.
††
Yes indicates that bacterial population count was performed after inoculation of plants; data are shown in Fig. 2.