Table 3.
Pst DC3000-resistant lines with zero or more than one cDNA insert
| Line No | Original line | Sequenced region | Accession no. | RAP ID | RAP description* | Response to C. higginsianum† |
|---|---|---|---|---|---|---|
| No cDNA insert (eight lines) | ||||||
| 36 | K20031 | S | ||||
| 37 | K31235 | S | ||||
| 38 | K37936 | S | ||||
| 39 | R05434 | S | ||||
| 40 | R06639 | S | ||||
| 41 | R06746 | S | ||||
| 42 | K30208 | S | ||||
| 43 | K30718 | S | ||||
| cDNA fragment chimera (based on full sequence) (1 line) | ||||||
| 44 | K02809 | 5′ | AK071280 | Os10t0110800-02 | Similar to Nitrate transporter (Fragment) | R |
| 3′ | AK071222 | Os10t0539400-01 | Similar to MCE-family protein MCE2C. | |||
| Single PCR fragment but unreadable (two lines) | ||||||
| 45 | K29426 | S | ||||
| 46 | R06632 | S | ||||
| Two independent cDNA inserts (4 lines + 2 independent lines‡) | ||||||
| 47 | R05622 | |||||
| A | 5′, 3′ | AK111700 | Os01t0113800-01 | Protein kinase, core domain containing protein. | S | |
| B | 5′, 3′ | AK103090 | Os01t0958100-02 | Similar to Chloroplast SRP receptor cpFtsY precursor. | ||
| 48 | K26225 | |||||
| A | 5′, 3′ | AK068060 | Os12t0605400-01 | Similar to CROC-1-like protein (Fragment). | S | |
| B | 5′, 3′ | AK065583 | Os10t0438600-01 | Similar to Family II lipase EXL3. | ||
| 49 | R07445 | |||||
| A | 5′, 3′ | AK102786 | None (Similar to Os05t0440250-01) | Similar to Histone deacetylase superfamily protein. | S | |
| B | 5′, 3′ | AK071245 | Os03t0608800-01 | PDZ/DHR/GLGF domain containing protein. | ||
| 50 | K17801 | |||||
| A | 5′, 3′ | AK066933 | Os06t0178900-01 | Vacuolar H+-pyrophosphatase | S | |
| B | 5′, 3′ | AK101159 | Os04t0528400-01 | Similar to ABC transporter. | ||
| Two independent inserts, more than two cDNAs in one insert (one line) | ||||||
| 51 | K35251 | |||||
| A | 5′ | AK073249 | Os05t0456900-01 | Conserved hypothetical protein. | R | |
| 3′ | AK067396 | Os01t0368700-01 | Protein of unknown function DUF679 family protein. | |||
| B | 5′, 3′ | AK105874 | Os01t0268800-01 | Ubiquitin-associated/translation elongation factor EF1B | ||
| More than two cDNAs at one insertion locus (total length of cDNAs at 5′ and 3′ shorter than the observed PCR fragment) (eight lines) | ||||||
| 52 | K02851 | 5′ | AK071506 | Os03t0383600-01 | Thiolase-like, subgroup domain containing protein. | S |
| 3′ | AK065536 | Os09t0244200-01 | Conserved hypothetical protein. | |||
| 53 | K37919 | 5′ | AK073569 | Os07t0175600-01 | Plant lipid transfer protein and hydrophobic protein | S |
| 3′ | AK067825 | Os03t0201600-02 | Similar to ischaemia/reperfusion inducible protein. | |||
| 54 | K35008 | 5′ | AK102635 | Os06t0273800-01 | Similar to Signal peptidase 18 subunit (Fragment). | S |
| 3′ | AK067216 | Os01t0868200-01 | Zinc finger, DHHC-type domain containing protein. | |||
| 55 | K36312 | 5′ | AK072635 | Os02t0532900-02 | Similar to H0717B12.10 protein. | S |
| 3′ | AK071613 | Os12t0555500-01 | Probenazole-inducible protein PBZ1. | |||
| 56 | K41633 | 5′ | AK072747 | Os04t0657100-01 | Similar to Farnesyl diphosphate synthase (Fragment). | S |
| 3′ | AK102417 | Os12t0540900-01 | Similar to Tryptophanyl-tRNA synthetase | |||
| 57 | R05917 | 5′ | AK100760 | Os12t0123600-01 | Similar to Nucleoside-triphosphatase | S |
| 3′ | AK069004 | Os03t0220700-02 | Peptidase, trypsin-like serine and cysteine domain containing protein. | |||
| 58 | R05945 | 5′ | AK065044 | Os03t0749300-01 | Similar to Exoglucanase precursor. | S |
| 3′ | anti-sense of AU069314 (vector fragment) | |||||
| 59 | R06201 | 5′ | AK064875 | Os03t0369800-01 | Similar to Novel plant SNARE 13 (AtNPSN13). | S |
| 3′ | AK071002 | Os12t0518000-01 | Hypothetical conserved gene. | |||
*
Predicted protein annotation provided by RAP-DB (http://rapdb.dna.affrc.go.jp/).
†
Colletotrichum higginsianum on the FOX lines; R, resistant; S, susceptible.