Skip to main content
. 2011 Mar 16;2011:846716. doi: 10.4061/2011/846716

Figure 4.

Figure 4

Effect of ERK1/2 inhibition on the STAT3 phosphorylation and DNA binding activity. (a) Western blot analysis demonstrates that STAT3 activation at 2 hours after ischemic insult is increased in normothermic group (37, n = 6). This increase was reduced by hypothermia (33, n = 6). In hypothermic group, U0126 treatment (33, n = 4) enhanced STAT3 phosphorylation to the level of normothermia group (37, n = 6). *P < .05 versus sham; #P < .05 versus hypothermic ischemia without U0126. (b) DNA binding activity assay of STAT3 using nuclear fraction from ischemic brain under hypothermia condition shows increased binding activity of STAT3 at 2 hours after MCAO (n = 4). STAT3 binding was further augmented by U0126 treatment (n = 4). *P < .05 versus sham; #P < .05 versus ischemia without U0126.