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Nonviable ED6 embryos, showing cavitation but either no or highly abnormal ICMs, initial outgrowths, and typical colony appearance. Brightfield images of embryos used for CU1 (a) and CU2 (b) hESC derivation. Phase contrast images of representative initial outgrowths two (c), five (d), or 14 days (e) postplating and typical established hESC colonies (f, g, h). Scale bar = 20 μm (a, b); 100 μm (c–h).
