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Gene expression analysis by qRT-PCR. Expression of pluripotency markers POU5F1/OCT4 (a), SOX2 (b), NANOG (c) and differentiation markers BRACHYURY (e), AFP (f), and PAX6 (g) in undifferentiated CU1, CU2, and CHB1 hESC colonies (hESC) and corresponding embryoid bodies (EmB) harvested on days 4, 8, 14, and 21 (EmBd4, EmBd8, EmBd14, and EmBd21, resp.). Expression levels were normalized to expression of GAPDH.
