Figure 2.

Sucrose density analysis of urine derived microvesicles for RNA content. (A) Urinary microvesicles were separated using sucrose density gradient centrifugation. The fractions were harvested, exosomal RNA was isolated and used for cDNA synthesis following RT-PCR analysis. (B) Isolated exosomes were incubated with RNase A alone or in combination with sonication to destroy the exosomal membranes. Isolated esRNA was analyzed by PCR. (C) Total RNA was isolated from amniotic fluid and urine exosomes and analyzed via an Agilent Bioanalyzer. The results show that exosomes contain variable amounts of 18 and 28S rRNAs as well as small and large RNAs.