Figure 3.

Western blots showing the relative expression of the AdK-L and AdK-S short isoforms in various mutant cell lines. Equivalent amount of cell extract (40 μg) from each of the cell lines was applied on the gels prior to electrophoresis and blotting. The WT refers to the parental V79 cells. VF and VT refer to various mutants selected using FoA and tubercidin, respectively. The results for the CHO cells and some of its mutant are present in the bottom right hand panel. Similar results for different mutants were obtained in at least two-independent experiments.