Table 1.
Degree of Resistance of Mutants Cell Lines to Adenosine Analogs
| V79 Cell Line | Relative Resistance of the Mutant Cell lines | |
|---|---|---|
| Formycin A | Tubercidin | |
| WT (A) | 1 (~20 ng/ml) | 1 (~10 ng/ml) |
| TubR2 (A) | >50 | >50 |
| TubR3 (A) | >50 | >50 |
| TubR4 (A) | >50 | >50 |
| TubR5 (A) | >50 | >50 |
| FomR2 (A) | >50 | >50 |
| FomR3 (A) | >50 | >50 |
| FomR4 (A) | >50 | >50 |
| FomR5 (A) | 50 | >50 |
| FomR6 (A) | >50 | >50 |
| FomR7 (A) | >50 | >50 |
| FomR8 (A) | >50 | >50 |
| FomR20 (A) | >50 | >50 |
| FomR24 (A) | >50 | >50 |
| FomR26 (A) | >50 | >50 |
| FomR1 (B) | 25 | 2 |
| FomR9 (B) | 50 | 5 |
| FomR10 (B) | 50 | 5 |
| FomR11 (B) | 50 | 5 |
| FomR12 (B) | >50 | 5 |
| FomR13 (B) | 25 | 2 |
| FomR15 (B) | 50 | 2 |
| FomR18 (B) | 10 | 1 |
| FomR19 (B) | >50 | 2 |
| FomR23 (B) | 50 | 2 |
| WT (CHO) | 1 | 1 |
| ToyR-4 (CHO) | >100 | >100 |
| DrToyR-18 (CHO) | >100 | >50 |
| FomR-4 (CHO) | 1.5 | >70 |
The degree of resistance of various cell lines towards formycin A and tubercidin was determined as described in Materials and Methods. Assuming the D10 values of these drugs for WT V79 cells as 1, the relative resistance of the mutant cell lines was calculated. (A) and (B) refer to the two class of mutants of V79 cells.