Skip to main content
. 2011 Jun 10;12(1):78. doi: 10.1186/1465-9921-12-78

Figure 2.

Figure 2

BrdU impairs epithelial regeneration after repeated NA exposure in mice. Mice were injected with NA once a week for 3 weeks (days 0, 7, and 14), and each NA injection was followed by administration of BrdU on 3 consecutive days. The animals were sacrificed on day 28, and lung tissue sections were double immunostained for (A) CC10 (green) and β-tubulin (brown), (B) CC10 (green) and Ki-67 (brown), and (E) BrdU (green) and Ki-67 (brown). Arrowheads indicate CC10-positive cells that express Ki-67. Arrows indicate cells that stained positive for BrdU. Broken arrows indicate cells that express Ki-67. (C and D) Quantitative analyses of the number of CC10-positive cells within the distal airway epithelium (C), and the proportion of CC10-positive cells that express Ki-67 and the proportion of CC10-negative cells that express Ki-67 (D). Data are expressed as the means ± SEM. N = 4-6 in each group of mice. BM: basement membrane. Panel E shows that very few BrdU-positive cells (green) stained positive for Ki-67 (brown).