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. 2011 May 27;8:261. doi: 10.1186/1743-422X-8-261

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Copyright ©2011 Barros et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Amplification of the envelope gene (env). Amplification of the Yellow Fever env gene by RT-PCR. (A) Agarose gel (0.8%) eletrophoresis showing the RT-PCR amplification product (lane 2) using the oligonucleotides YFE2460R e YFE878F. Lane 1 shows the molecular mass marker, 1 Kb plus DNA ladder (Invitrogen). (B) Diagram of the Yellow Fever genome (Genebank accession # YFU17066 ) showing the main ORF (polyprotein ORF) and the position of the structural (Cap, pM and E) and non-structural (NS1, NS2a, NS2b, NS3, NS4a and NS5) proteins. The position of the oligonucleotides YFE2460R e YFE878F, used for the amplification reaction are also shown.