Figure 3.

E protein detection of vSynYFE-infected insect cells by fluorescence microscopy. Tn5B cells were infected with vSynYFE (10 pfu/cell) and at 72 h p.i., cells were fixed with ice cold acetone and incubated with a polyclonal anti-FLAV antibody (conjugated with fluorescein isothiocyanate) and viewed in a fluorescence microscope. (A) Tn5B cells mock infected (bright field). (B) Tn5B cells infected with vSynYFE showing multinucleated syncytial cells (bright field). (C) Tn5B cells mock infected (fluorescence). (D) Tn5B cells infected with vSynYFE showing multinucleated syncytial cells (fluorescence). In A, B, C and D, Bars = 30 μm.