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. 2011 May 27;8:261. doi: 10.1186/1743-422X-8-261

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Copyright ©2011 Barros et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Dengue MAC-ELISA assay. In this modified IgM antibody capture ELISA (MAC-ELISA) assay a pool of sera from dengue virus-infected patients [containing antibodies against the 3 DENV sorotypes 1 (D1), 2 (D2) and 3 (D3)] were used with the following antigens: mock infected Tn5B cells, Tn5B cells infected with AcMNPV and Tn5B cells infected with vSynYFE. Positive control antigen is a pool of dengue virus (D1D2D3) and CN is the same assay with serum from a healthy individual. No reactivity was detected with vSynYFE-infected insect cells extracts treated with dengue-infected patients sera.