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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Nat Struct Mol Biol. 2011 Mar 27;18(4):423–431. doi: 10.1038/nsmb.2038

Figure 6. ABC-ATPase superfamily conserved basic-switch residues in Rad50 coordinate DSBR in S. pombe.

Figure 6

(a) Rad50 sequence alignment with ABC transporters shows the extended signature motif, with well-conserved residues shaded. Red circles (point mutations) and triangles (truncations) denote cystic fibrosis causing CFTR mutations. (b) S. pombe Rad50 basic-switch variants on the signature helix are defective for DSBR. Left: Expression levels of TAP-tagged Rad50 variants, as probed by PAP antibody. Right: IR, CPT, HU and UV sensitivity of Rad50 basic-switch variants. Five-fold serial dilutions of cells on rich media plates were photographed following 2–3 days at 30°C. Strains used are detailed in Supplementary Table 1.