Figure 3.

JM6 prevents spatial memory loss and anxiety deficits in a mouse model of AD. (A) JM6 rescues spatial memory deficits in APPtg mice in the Morris water maze (MWM). Mice were trained to find a hidden platform in the MWM using spatial cues. Spatial memory was assessed 24 h after training by removing the hidden platform and quantifying how much time mice spent in the target quadrant where the platform had been placed previously. APPtg mice that received JM6 (75 mg/kg/day p.o. for 120 days) spend significantly more time in the target quadrant than vehicle-treated controls. Solid bar, target quadrant; white bar, average of three other quadrants. (B) JM6 reduces anxiolytic behavior in APPtg mice in the elevated plus maze (EPM). APPtg mice are disinhibited and spend an equal amount of time in the open and closed arms in the EPM. However, APPtg mice that receive JM6 spend significantly more time in the closed arm than in the open arm and are not significantly different from WT mice. Values are means ± s.e.m. *p<0.05, **p<0.01, ***p<0.001. For (A,B), t tests were used to calculate differences between target vs. other quadrants, and time spent in open vs. closed arm, respectively (n = 8–14 mice per group). One-way ANOVA was used to calculate differences among the four experimental groups. JM6 reduces the distance traveled by APPtg mice in the open arm of the elevated plus maze. (C,D) APPtg mice that receive JM6 (75 mg/kg/day p.o. for ~120 days starting at day 30) travel significantly less distance in the open arm of the elevated plus maze than vehicle-treated controls, but the total travel distance in both arms of the maze is not different. Values are means ± s.e.m. ***p<0.001, t-test, open vs. closed arm. **p<0.01, ***p<0.001, one-way ANOVA comparing open arms between groups (n = 8–14 per group). ns: not significant.