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Expression of CRF receptor protein in heart tissue decreased with increased temperature. Protein extracts from the heart tissue of one naïve rat was incubated with antibody at 37°C, 65°C or 95°C. Labeling of CRF1/2 (A) and CRF2 (B) protein showed temperature dependence. Note the presence of tissue-specific CRF2 labeling and higher oligomers of CRF1 at lower temperatures.
