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. Author manuscript; available in PMC: 2011 Jun 20.
Published in final edited form as: Eur J Immunol. 2009 Apr;39(4):1147–1156. doi: 10.1002/eji.200839184

Figure 5.

Figure 5

Inhibition of IgM binding to hFcα/μR by anti-IgM-Fc-specific Ab. (A) IgM binding to fixed and permeabilized COS-7 cells transfected with human FcαμR can be inhibited by a 1:1000 dilution of mAb 4–3 (anti-Cμ3). Similar results were obtained with mAb 1G6 (anti-Cμ4). (B) Erythrocytes opsonized with IgM (~133 μg/mL) and pre-incubated with 100 μg/mL of either anti-IgM Fc mAb 4–3 or 1G6 fail to rosette with hFcαμR transfectants. Figure shows the mean±SD of two independent experiments. Differences between the untreated control group and erythrocytes pre-incubated with mAb 4-3 or 1G6 were analyzed using Dunnett’s multiple comparison test. Statistically significant differences between the groups are indicated.