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. Author manuscript; available in PMC: 2012 Jun 2.
Published in final edited form as: Psychosom Med. 2011 Jun 2;73(5):393–400. doi: 10.1097/PSY.0b013e31821df0c2

Figure 2.

Figure 2

Oxytocin immunoreactivity in plasma and plasma extracts after gel filtration chromatography. Plasma (400 μL, A and C) or extracts of the same samples (B and D) were applied to a Superdex 75 10/300 GL column and eluted with 5-mM ammonium acetate buffer (pH 7.8) at a flow rate of 1.0 mL/min, and 1.5-mL fractions were collected. After collection, the fractions were lyophilized then reconstituted and assayed for oxytocin immunoreactivity by EIA. Samples 1 and 2 had plasma oxytocin levels of 442 and 281 pg/mL, respectively, and after extraction, 15.2 and 6.1 pg/mL, respectively. Elution of MW markers and of oxytocin is indicated above A and C.