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Western blotting of NRIF protein expression in shRNA-transfected Neuro2a cells. Control non-silencing shRNA- and NRIF-shRNA-transfected Neuro2a cells were cultured for 5 days, lysed, immunoprecipitated with NRIF antibody and analyzed by Western blotting. The blot was probed with anti-NRIF antibody. While control cells express NRIF protein, NRIF-shRNA-transfected cells do not show any NRIF protein expression
