NRIF-shRNA expressing cells show altered expression of lipid transcripts in both cholesterol-deficient and regular medium. The experiment was performed with the same parameters as described in Fig. 4. Note that all tested lipid system-related transcripts (HMGCR, DHCR7, FASN, SREBP2, SCAP, S1P, and SQS1) showed significantly reduced expression in NRIF-shRNA-transfected cultures compared to cells transfected with non-silencing shRNA, regardless if the cells were grown in regular or cholesterol-deficient cell culture media