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. Author manuscript; available in PMC: 2011 Jun 20.
Published in final edited form as: J Biol Chem. 2003 Jul 16;278(38):36358–36364. doi: 10.1074/jbc.M306045200

Fig. 2. Time- and concentration-dependent inactivation of SULT1A1 by 2,3-butanedione.

Fig. 2

A, log(percent activity) versus time. SULT1A1 (0.1 mg/ml) was incubated with different concentrations of 2,3-butanedione at room temperature in 50 mM borate, 1 M NaCl, pH 7.5. Aliquots were taken at different time points. SULT1A1 remaining activity was assayed using the PNPS assay method in a mixture containing 0.5 M arginine. B, log(Kapp) versus log(BD).