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. Author manuscript; available in PMC: 2011 Jun 20.
Published in final edited form as: J Biol Chem. 2003 Jul 16;278(38):36358–36364. doi: 10.1074/jbc.M306045200

Table I.

Primer pairs for SULT1A1 mutants

Mutantsa Primers
R78A 5′-GCTCCCATCTTCATGGCCGTGCCCTTCC-3′
R78E 5′-GCTCCCATCTTCATGGAGGTGCCCTTCC-3′
R78r 5′-GGAAACTGCCACATCCTTTGCGTTGCGG-3′
R130A 5′-GGTCTATGTTGCCGCCAACGCAAAGGATGTGC-3′
R130E 5′-GGTCTATGTTGCCGAGAACGCAAAGGATGTGC-3′
R130r 5′-GGTAGAGAACAGGGTGGGTCCGGCTCAGCTCC-3′
R257A 5′-GCATCTCCCCCTTCATGGCCAAAGGCATGGCT-3′
R257E 5′-GCATCTCCCCCTTCATGGAGAAAGGCATGGCT-3′
R257r 5′-CGCTTGGTCAGGTTTGATTCGCACACTCCCTC-3′
R213E 5′-CCTGGAGTTTGTGGGGCAATCCCTGCCAGAC-3′
R213r 5′-GGACCTCAAACACCCCGTTAGGGACGGTCTC-3′
a

Note: two different mutants in the same position used the same reverse primer (r).