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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Glia. 2011 Feb;59(2):267–277. doi: 10.1002/glia.21099

Figure 2. ADSCs are localized outside the sciatic nerve parenchyma, but induce changes within the nerve parenchyma.

Figure 2

Mutant sciatic nerves were treated with media (DMEM, A) or 5 × 104 CM-DiI labeled 3T3/L1 cells (B) or ADSCs (C). Few DiI-labeled 3T3/L1 cells (red, arrows) were detected (B), whereas many DiI-labeled ADSCs (red, arrows) were detected throughout the perineurium (C). Nuclei are stained blue with DAPI. Media- and ADSC-treated mutant limbs were analyzed by immunfluoresence for expression of the neural cell adhesion molecule L1 (D–F) which is not expressed in mutant sciatic nerves (Yu et al. 2009b). DMEM-treated sciatic nerves minimally expressed L1 (white) (D). One day after ADSC-treatment, there was significant increase in L1 immunostaining (white, arrows) (E), which persisted through post-operative day 21 (F). Three mice at each time point were evaluated, and these images are representative of these results.