Skip to main content
. Author manuscript; available in PMC: 2011 Jun 20.
Published in final edited form as: Thorax. 2010 Apr;65(4):346–353. doi: 10.1136/thx.2008.101139

Figure 4. Recovery of iNOS function after SPR activation correlates with the decrease in SPR-mediated synthesis of the inducible Hsp70 protein in alveolar macrophages.

Figure 4

iNOS function recovers 48 hours after SPR activation. MH-S cells were incubated at 43°C for 30 min, then allowed to recover at 37°C for the indicated times prior to IFN-γ stimulation (10 ng/ml) for 24 hours. Extracellular medium was assayed for nitrite using the Griess reagent. Results are the mean ± SD of 4 separate experiments with each condition carried out in triplicate; *p < 0.05 from control cells; **p < 0.05 from cells treated with IFN-γ alone; #p < 0.05 from cells heat stressed/recovered 1 hour and treated with IFN-γ.