Cell motility measured as a function of EGF concentration gradients G0 through G5 15 hours post-steady-state: G0=0, 0<G1<10−3 ng/mL per mm, 10−3<G2<10−2 ng/mL per mm, 10−2<G3<10−1 ng/mL per mm, 10−1<G4<100 ng/mL per mm, and 100<G5<101 ng/mL per mm of the microchannel. Cell motility was defined as the directed distance traveled by each cell divided by time. The number of cells per experiment is shown in parentheses.