Skip to main content
. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Anal Biochem. 2011 Mar 29;415(1):52–58. doi: 10.1016/j.ab.2011.03.029

Figure 1. Tissue preparation for Ziptip® assay with mass spectrometer interface.

Figure 1

A 500 micron biopsy punch is taken from a 1 mm spinal cord slice, thawed in buffer and adsorbed onto a Ziptip. Solvent from an HPLC pump flowed through 0.005 inch interior diameter PEEK tubing that was inserted snugly into the back of the Ziptip. The end of the Ziptip was inserted into a second short segment of PEEK tubing with an interior diameter of 0.03 inches that had been slightly expanded with a dissecting needleto fit the end of theZiptip snuggly. Astainless steel1 μm microfilterwas inserted between the Ziptip and the mass spectrometer inlet to trap large particulates released from the Ziptip.