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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Anal Biochem. 2011 Mar 29;415(1):52–58. doi: 10.1016/j.ab.2011.03.029

Figure 4. Stability of metal binding of G93A SOD1 in elution solvent.

Figure 4

A. Chromatogram of G93A SOD1eluting from the Ziptip. B. Parent mass spectrum of G93A SOD1mixed immediately in elution solvent and directly injected into the mass spectrometer. C. Parent mass spectrum of G93A SOD1 incubated in elution solvent for 5 minutes to mimic the maximum exposure of SOD1 to solvent when bound to a Ziptip. *In both B and C, the sulfate-bound SOD1 peak was more prominent probably due to the absence of the washing step used with the Ziptip.