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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Anal Biochem. 2011 Mar 29;415(1):52–58. doi: 10.1016/j.ab.2011.03.029

Figure 5. Metal content of G93A SOD1 in transgenic rats with symptoms of motor neuron degeneration.

Figure 5

A.Deconvoluted mass spectrum of a ventral tissue punch from the lumbar spinal cord of a G93A SOD1-overexpressing rat shown over the mass range 8000 to 24000 Da. Several other proteins, including ubiquitin, ubiquitin minus two glycine residues (-114 Da), and acyl-CoA binding protein were observable in addition to SOD1. B. Overlay of the mass spectra of dorsal and ventral tissue punches from the lumbar spinal cord of a G93A SOD1 overexpressing rat. Prominent peaks are identified in the spectrum, including the endogenous rat SOD1.C. Western blot of tissue punch supernatant (S) and pellet (P) fractions used to verify the release of SOD1 into the supernatant. The SOD1 standards were 4, 2, and 1 pmol, respectively. D. Standard curve using bovine SOD1 as an internal standard at a concentration of 800 nM and varying the G93A SOD1 over a concentration range of 200 nM-1.2 μM. The ratio of the peak integrations of the G93A SOD1 over the bovine SOD1 was plotted against the amount of G93A SOD1 in the sample to produce the standard curve.