Figure 1. All Akt isoforms were expressed in primary astrocytes.
PMAs were isolated from p53cKO or PtencKO;p53cKO mice with or without Akt1 deletion (Akt1 KO) and transduced with control (GFP) or EGFRvIII (vIII) expressing retrovirus. (A) Immunoblots were performed with the indicated antibodies. (B) Phospho-Akt (S473) immunoprecipitates were immunoblotted as indicated.