Figure 5. Transformed PMAs formed high-grade astrocytoma following orthotopic transplantation.
(A) Kaplan-Meier curves of mice following implantation of p53cKO;EGFRvIII PMAs with or without Pten deletion. (B) IHC analysis of representative tumors from (A). Sections were stained for human EGFR (shown at low and high magnification), Pten, pAkt (S473), Gfap and Nestin. (C) Left: IHC for Ki67 in p53cKO;EGFRvIII tumors with or without Pten. Right: Quantification of Ki67 positive cells. Shown is the mean ± SEM. Bars in A and C are 100 μm.