Figure 6. Loss of Akt1 and Akt2 had opposing effects on latency of Pten deficient tumors.

Kaplan-Meier curves of mice following intracranial implantation of (A) Pten^WT;p53^cKO;EGFRvIII or (B) Pten^cKO;p53^cKO;EGFRvIII PMAs that were (upper) Akt1 WT or KO, or treated with (middle) Akt2- or (lower) Akt3-shRNAs. (C) Left: IHC for Ki67 (brown) was performed on Pten^cKO;p53^cKO;EGFRvIII tumors from (B, upper) and counterstained with hematoxylin (blue). Bar is 100 μm. Right: Quantification of Ki67 positive cells. Shown is the mean ± SEM. The significance value (p) was determined using an unpaired t test. (D) Kaplan-Meier curves of mice following intracranial implantation of (left) Pten^WT;p53^cKO;EGFRvIII or (right) Pten^cKO;p53^cKO;EGFRvIII PMAs that were Akt1^WT (black), Akt1^KO (green) or Akt1^KO expressing (blue) Akt2 or (red) Akt3 shRNAs. For A, B and D, n=number of mice per group and p values were calculated using the logrank test.