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A: pulsatile GnRH release increases significantly during treatment with the nonselective PKA inhibitor H-89. B: treatment with H-89 also markedly increases pulsatile cAMP secretion. C: treatment of perifused GT1–7 neurons with selective cAMP-dependent PKA inhibitor cAMPS-R also increases pulsatile GnRH release. Shown are representative traces from 4 independent experiments.
