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IR substrate 1 (IRS1)-associated phosphatidylinositol 3-kinase (PI3K) activity in epitrochlearis and soleus muscles with 0, 1.2, or 30 nM insulin. ANOVA P values are as follows: for epitrochlearis, P = 0.630 (diet), P = 0.224 (insulin), and P = 0.838 (interaction); for soleus, P = 0.641 (diet), P < 0.001 (insulin), and P = 0.971 (interaction). Values are means ± SE; n = 6 muscles per diet group and insulin concentration.
