Fig. 5.

BrCr treatment decreases expression of milk protein and lipogenic genes in whole mammary gland. Standard curve method qRT-PCR was used to determine gene expression profiles for milk proteins CSN2 and WAP as well as for Glut1, mitochondrial citrate transporter (Slc25a1), de novo fatty acid synthesis enzymes Acly and Fasn, and regulatory factors Srebp1c and Akt1. Profiles are expressed as transcript copy number detected in 50 ng of total RNA relative to 0 time point and error bars are SE for 4 animals per time point. *Means differ significantly (P ≤ 0.05) from control.