Table 1.
Adipose-Depleted MECs |
|||
---|---|---|---|
Functional Category | Control | BrCr | Fold change |
Milk protein genes | |||
Csn2 | 1.43 | 0.86 | −1.65c |
Lalba | 1.36 | 0.67 | −2.01b |
Wap | 1.10 | 0.88 | N/C |
Glycolysis and pentose shunt | |||
Glut1 | 1.95 | 0.54 | −3.59c |
G6pd2 | 0.91 | 0.38 | −2.43b |
AldoC | 1.69 | 0.37 | −2.93b |
Gapdh | 1.24 | 0.55 | −2.25c |
Me1 | 1.09 | 0.50 | −2.16b |
De novo fatty acid synthesis | |||
Slc25a1 | 1.69 | 0.37 | −4.59a |
Acly | 1.65 | 0.30 | −5.45d |
Acc1α | 1.44 | 0.22 | −6.43d |
Fasn | 1.08 | 0.34 | −3.13b |
Thrsp | 0.77 | 0.69 | N/C |
TAG synthesis and β-oxidation | |||
Dgat1 | 1.08 | 0.48 | −2.24b |
Scd2 | 2.03 | 0.10 | −19.46c |
Fads1 | 1.44 | 0.40 | −3.62c |
Acaa2 | 0.50 | 0.52 | N/C |
Acadl | 0.46 | 0.63 | N/C |
Regulatory factors | |||
Akt1 | 2.55 | 0.92 | −2.77c |
Prlr-Long | 0.96 | 0.37 | −2.60d |
Srebp1a | 0.77 | 0.38 | −2.05b |
Srebp1c | 0.74 | 0.22 | −3.34b |
Gene expression in adipose-depleted mammary epithelial cells (MECs) following 8-h bromocriptine (BrCr) treatment. Genes are grouped into functional categories. Values are mean normalized transcript copy numbers for that gene using standard curve-based qRT-PCR; n = 5 mice per condition. Fold change is calculated as −1/(BrCr/Control). Statistical significance: aP ≤ 0.05, bP ≤ 0.01, cP ≤ 0.001, dP ≤ 0.0001. N/C, no statistical change, using Excel's t-test function.