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. 2011 Mar 8;300(6):E1047–E1058. doi: 10.1152/ajpendo.00666.2010

Fig. 7.

Fig. 7.

Mitochondrial bioenergetic adaptation to HFD feeding in isolated kidney mitochondria. Experiments were run on a SeaHorse XF24 extracellular flux analyzer to obtain bioenergetic profiles from LFD- and HFD-fed groups at 12 and 16 wk. OCR, oxygen consumption rate. A–E: typical bioenergetic parameters in the 12-wk feeding group. Some parameters showed improvement as an adaptation. A: respiratory control ratio (RCR). B: ATP-linked respiration. C: proton leak. D: nonmitochondrial respiration. E: basal respiration rates. F–J: same as A–E in the 16-wk feeding group. K: philosophy of the SeaHorse extracellular flux analyzer machine and interpretation of the various parameters. Data represent means ± SE for 2 independent cohorts (n = 8 per group). *P < 0.05 vs. LFD group.