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. 2001 Mar 13;98(7):4101–4106. doi: 10.1073/pnas.051013898

Figure 1.

Figure 1

A 72-nt-long DNA was synthesized in a DNA synthesizer. Two regions (see text) of the s1 RNA (nucleotides 523–561 and 973-1005) (22) were fused. Transcripts of this DNA possess two Rz cleavage sites, GUA and GUC (underlined). The cleavage is expected to take place downstream of the A and C nucleotides of the target RNA (shown by arrows). The sequences of the two Rzs, 553 and 984, along with the hammer-head catalytic motif are also shown. Both the Rzs possessed the earlier described hammer-head catalytic motif. Eight bases on either side of the target site were synthesized to provide specificity for the s1 RNA. For Rz-553 the A nucleotide and C nucleotide (shown by arrows) for Rz-984 of the target RNA was left unpaired. The S1–553-mutant-Rz was constructed by changing a single nucleotide (G to U) in the catalytic motif (see text).