Table 1.

Kinetic analysis of S1 Rzs

Enzyme	Kcat, min−1	Km, M	Kcat/Km, M−1⋅min−1
S1-553-Rz	30.3  × 10−4	1.8  × 10−7	1.68  × 104
S1-984-Rz	5.7  × 10−4	4.8  × 10−7	0.12  × 104

Kinetic measurements were carried out for the reovirus S1 Rzs by taking varying amounts of labeled s1-synthetic substrate RNA and carrying out cleavage reactions under Rz-saturating conditions as described before. The kinetic parameters were calculated from a Lineweaver–Burk's plot. Rate constants are averages of two separate experiments.