Fig. 9.

Effect of ALDO-A knockdown or overexpression on the level of gelsolin expression in HeLa cells. A: cells were transfected with aldolase-specific siRNA or aldolase cDNA to knockdown or overexpress aldolase. Levels of protein expression were analyzed by Western blotting. The same blot was probed with antibodies for ALDO-A (top), gelsolin (middle), and actin as a loading control (bottom). B: densitometric quantification of the levels of expression of ALDO-A and gelsolin protein in HeLa cells. Numbers correspond to the intensities of the bands relative to the negative control siRNA-transfected cells (Neg siRNA). EGFP, enhanced green fluorescent protein; DsRed, monomeric red fluorescent protein. Values are shown in the same order as the bands presented in A.