Fig. 5.

Effect of ERK1/2 and p38 inhibition on lysosomal targeting of GFP-tagged full-length Prdx6 and a peptide comprising Prdx6 amino acid (aa) 31–40 in MLE12 cells. GFP-tagged or Prdx6(31–40 aa) peptide (A) Prdx6 full-length (FL) (B) fusion construct expressed in the transfected cells is shown in green (left). Lysosomal-like organelles are stained with Nile Red (middle). Colocalization is detected by yellow color in the merged image (right). Inhibitors were applied, where indicated, 1.5 h before fixation of transfected MLE12 cells.