Fig. 4.

Short hairpin (sh)RNA-mediated knockdown of Krp1 protein levels. A: Western blots for Krp1 and β-actin (loading control) in lysates of cells stably transfected with shRNA targeted to MBP (shMBP) or Krp1 (shK1 and shK2) and harvested after 72 h in DM. B: quantification of Krp1 protein levels assessed by Western blot as in A at the time points indicated in DM, and expressed relative to levels in the shMBP line. Data are means ± SE. *P < 0.05, significantly different from shMBP cells as assessed by t-test; n = 3 independent experiments.